ANNUAL MEETING POSTERS 5-04

1. Development of a Reaction-Based Signaling Map for the FXM. [jpeg] [ppt]
   Gil Sambrano, Lily Jiang, Madhu Natarajan, Alex Gilman, and Adam Arkin
2. Development of RNA Interference Reagents for Perturbation Studies in RAW 264.7 Cells. [jpeg] [ppt]
   Joelle Zavzavadjian, Leah Santat, Jamie Liu, Estelle Wall, Kavitha Dhandapani, Jong-Ik Hwang, Melvin Simon, and Iain Fraser
3. Lentiviral Vector Transduction of RAW 264.7 Cells for Stable shRNA-Mediated RNAi. [jpeg] [pdf]
   Tamara Roach, Robert Rebres, and Iain Fraser
4. Single-Cell Calcium Measurements Reveals Heterogeneity of Ligand Induced Responses in RAW 264.7 Cells. [jpeg 1, jpeg 2] [ppt]
   Mary Verghese, Michal Ronen, Elizabeth Gehrig, Bob Sinkovits, Joelle Zavzavadjian, Leah Santat, Jamie Liu, Estelle Wall, Kavitha Dhandapani, Iain Fraser, Robert Rebres, Tamara Roach, and Grischa Chandy
5. A Single-Cell PIP3 Assay Based on Akt-PH Domain Translocation in RAW 264.7 Cells. [jpeg] [ppt]
   Jim Whalen, Elizabeth Gehrig, Takako Mukai, WeiSun Park, Ilango Vadivelu, Joelle Zavzavadjian, Leah Santat, Jamie Liu, Estelle Wall, Kavitha Dhandapani, Iain Fraser, Robert Rebres, Tamara Roach, and Nancy O'Rourke
6. Localization, Translocation, and Binding Properties of Mouse PH Domains in RAW 264.7 Cells. [jpeg] [ppt]
   Takako Mukai, Jim Whalen, WeiSun Park, Elizabeth Gehrig, Ilango Vadivelu, Joelle Zavzavadjian, Estelle Wall, Andreia Maer, Grischa Chandy, and Nancy O'Rourke
7. Yeast 2-Hybrid Studies in AfCS Model Cell Systems. [jpeg] [ppt]
   Iain Fraser, Bob Sinkovits, Gil Sambrano, Terrece Pearman, Max Dufford, Keng-Mean Lin, Robert Hsueh, Tamara Roach, Karen Heichman, and Melvin Simon
8. Modeling Single Cell's Calcium Response. [ppt]
   Michal Ronen, Grischa Chandy, Tobias Meyer, and James Ferrell
9. Classification of the Localization Patterns of Tagged Kinases and Other Signaling Proteins in RAW 264.7 Cells. [jpeg] [ppt]
   James H. Whalen, Weisun Park, Mary Verghese, Elizabeth Gehrig, Joelle Zavzavadjian, Ilango Vadivelu, Estelle Wall, Kavitha Dhandapani, Andreia Maer, Brian Saunders, Nancy O' Rourke, Iain Fraser, and Grischa Chandy
10. Inhibition of Gene Expression by Antisense Oligonucleotides in RAW 264.7 Cells. [jpeg]
    Pamela Eversole-Cire, Joe Johnson, Iain Fraser, and Melvin Simon
11. Protein Phosphorylation Studies in RNAi-Mediated Gene Knock-Down Cells. [jpeg] [ppt]
    Yan Ni, Lily Jiang, Tamara Roach, Robert Rebres, and Iain Fraser
12. The AfCS-NPG Molecule Pages - A Comprehensive Signaling Database. [jpeg] [ppt]
    Joshua Li, Yuhong Ning, Warren Hedley, Brian Saunders, Timo Hannay, Yongsheng Chen, Nicole Tindill, Robert Sinkovits, Ron Taussig, and Shankar Subramaniam
13. The AfCS Membership and Editorial Office: Molecule Pages Plus. [jpeg] [ppt]
    Sharon Brown, Ashley Butler, and Patrick Casey
14. Monitoring Protein Phosphorylation: Comparison of Western Blotting and Bio-Plex. [jpeg] [ppt]
    Heping Han, Biren Zhao, Claudia Suen, and Judith Zhu-Shimoni
15. Simultaneous Detection of Multiple Phosphorylated and Total Proteins Using the Bio-Plex Suspension Array System. [jpeg] [pdf]
    Qian Gao, Kris Simonyi, Claudia Suen, Judy Shimoni, and Sophie Allauzen
16. Identification of Ligand Responses for the AfCS Ligand Screen in RAW 264.7 Cells. [jpeg] [ppt]
    Robert Hsueh, Keng-mean Lin, Ruth Levitz, and Heping Han
17. Boolean Network Inference and Experiment Design for the B-Cell Ligand Screen. [jpeg] [ppt]
    Stuart Johnson
18. Visualizing Protein Phosphorylation on a Signaling Map. [jpeg] [ppt]
    Sylvain Pradervand
19. Macrophage Ligand Screen: Phosphoproteins. [jpeg 1, jpeg 2] [ppt]
    Nicholas Wong, Robert Hsueh, Robert Sinkovits, and Heping Han
20. AfCS Antibody Database: New Web-based Version. [jpeg] [ppt]
    Becky Fulin, Lonnie Sorrells, Robert Sinkovits, Ruth Levitz, and Heping Han
21. Lipidomic Profiling of RAW 264.7 Macrophages: the FXM ligands. [ppt]
    Jeffrey Forrester, Stephen Milne, Pavlina Ivanova, Michelle Armstrong, and Alex Brown
22. Multiplexed Lipid Arrays of the AfCS Ligand Screen. [ppt]
    Stephen Milne, Jeffery Forrester, Pavlina Ivanova, Michelle Armstrong, and Alex Brown
23. Mass Spectrometry Analysis of Phosphatidylinositol Phosphates. [ppt]
    Pavlina Ivanova, Stephen Milne, Jeffrey Forrester, Michelle Armstrong, and Alex Brown
24. Macrophage Ligand Screen: Cytokines. [jpeg] [ppt]
    Ruth Levitz, Robert Hsueh, Lonnie Sorrells, Robert Sinkovits, Heping Han, and Ron Taussig
25. Transcriptional Regulation of Pathways Activated by Lipopolysaccharide in RAW 264.7 Cells. [jpeg] [ppt]
    Sangdun Choi, Yun Anna Cao, Sun Young Lee, Mi Sook Chang, Xiaocui Zhu, Meghdad Rahdar, Robert Hsueh, Paul Sternweis, and Melvin Simon
26. Gene Expression Profiling Reveals Interactions of Signals Activated by Different Proliferative Ligands in B Cells. [jpeg]
    Xiaocui Zhu, Yun Anna Cao, Sun Young Lee, Mi Sook Chang, Meghdad Rahdar, Robert Hsueh, Paul Sternweis, Sangdun Choi, and Melvin I. Simon
27. Ligand Screen for Calcium Assays in Primary B Cells and RAW264.7 Cells. [jpeg 1, jpeg 2] [ppt]
    Keng-Mean Lin, Madhusudan Natarajan, Robert Hsueh, and Paul Sternweis
28. Ligand Screen for cAMP Assays in Primary B Cells and RAW264.7 Cells. [jpeg 1, jpeg 2] [ppt]
    Keng-Mean Lin, Robert Hsueh, Madhusudan Natarajan, and Paul Sternweis
29. B-cell Double Ligand Microarray Data Analysis — Data Visualization and Interpretation. [pdf]
    Prashanth Vishwanath, Gabriel Eichler, and Temple Smith
30. Model-based Analysis of Single- and Double-ligand Calcium Response Screening Data for Macrophage. [jpeg] [pdf]
    Tomomi Kimura, Kanae Oda, Yukiko Matsuoka, Masaaki Muramatsu, and Hiroaki Kitano
31. Endocytosis Pathway Activation by Signaling through the B cell Antigen Receptor Revealed by a Tool for the Functional Classification of Microarray Gene Clusters. [jpeg] [ppt]
    Jamie A. Lee, Robert Sinkovits, Dennis Mock, Eva Rab, Jennifer Cai, Peng Yang, Brian Saunders, Robert Hsueh, Sangdun Choi, Tamara Roach, Shankar Subramaniam, and Richard Scheuermann
32. Microarray Analysis: Context-dependent Signaling in RAW 264.7 Cell Line. [jpeg] [ppt]
    Dennis Mock, Yi-Xiong Zhou, Jing-Wei Meng, and Shankar Subramaniam
33. Mouse Genome Informatics: A Mouse Functional Annotation Central. [pdf]
    T.B.K. Reddy, Joel Richardson, Janan Eppig, Martin Ringwald, Carol Bult, James Kadin, Judith Blake
34. Towards a Signal Transduction Network of the Living Cell.
    Stephen Michnick, France Croze, Ingrid Remy, Annie Montmarquette, Brigitte Keon, and John Westwick
35. A Cytometric Assay for Macropinocytosis in RAW 264.7 Cells. [jpeg] [pdf]
    Robert Rebres and Tamara Roach
36. Identification and Validation of a PKA Substrate in RAW 264.7 Cells. [jpeg] [ppt]
    Farah El Mazouni, Qun B, Laura Draper, Kathleen Lyons, Deepa Sethuraman, Hongjun Shu, and Deirdre Brekken
37. Building a Database of Phosphoproteins Present in RAW 264.7 Cells. [jpeg] [ppt]
    Deirdre Brekken, Qun Bi, Kathleen Lyons, Deepa Sethuraman, and Hongun Shu
38. Quantitative Analysis of Protein Phosphorylation in RAW 264.7 Cells Using SILAC. [jpeg] [ppt]
    Honjun Shu, Qun Bi, Robert Cox, Laura Draper, Farah El Mazouni, Kathleen Lyons, Marc Mumby, Deepa Sethuraman, and Deirdre Brekken